A Method for Applying Haemolysates on Starch Blocks for Electrophoresis.
نویسندگان
چکیده
Electrophoresis of haemolysates on a starch block, which was first described by Kunkel and Wallenius (1955), provides an excellent method for separating and quantitating the various normal and abnormal haemoglobins. Since relatively large amounts of haemoglobin solutions can be applied it is a useful preparative method for isolating a particular haemoglobin for further analysis. One of the important requirements for good electrophoretic resolution on the block is the application of the sample in a narrow band and a simple method for applying the haemolysate is described.
منابع مشابه
Re-evaluation of the presence of multiple haemoglobins during the ontogeny of the mouse.
In mice, three alleles, Hbbs, Hbbd and Hbbp, have been found at the beta-chain locus of haemoglobin. Analytical polyacrylamide gel electrophoresis revealed the presence of almost the same four haemoglobin bands (E-I, E-II, E-III and X) in all of the lysates of peripheral blood cells from 12-day-old foetuses of C57BL/6J (Hbbs), A/He (Hbbd) and PON (Hbbp). All were embryonic-type haemoglobins; th...
متن کاملA Novel Method to Detect β-Cyclodextrin Glucosyl Transferase (β-CGTase) Activity on Polyacrylamide Gels
b-cyclodextrin glucosyl transferase (b-CGTase) hydrolyses starch to produce b-cyclodextrin by transglycosylation (cyclization) activity. The conventional method for detection of b-CGTase activity is based on detecting starch hydrolysis by iodine staining. This method reveals all amylolytic enzymes, but can not discriminate them. In the present study, we introduce a new method for specific detec...
متن کاملThe transition from tadpole to frog haemoglobin during natural amphibian metamorphosis. II. Immunofluorescence studies.
Rabbits were immunized with frog or tadpole haemoglobin purified either by chromatography or by polyacrylamide gel electrophoresis. The obtained rabbit antisera were shown to be specific for frog or tadpole haemolysates by double diffusion, immunoprecipitation and immunonuorescence. Indirect immunofluorescent staining of peripheral blood smears of Rana catesbeiana tadpoles at the metamorphic cl...
متن کاملUsing starch powder for the decontamination of I-131 in isolation rooms
Introduction: Various iodine isotopes are undergoing radioactive decay except Iodine-127 (I-127. Iodine I-131 is unsealed radioactive material used in nuclear medicine for both diagnostic and therapeutic procedures in Royal Hospital since 2006. In radiotoxic classification, I-131 is in group 2 element. I-131 is highly reactive element due to its volatilization nature. Currently...
متن کاملA new method for starch gel electrophoresis of human hemoglobins, with special reference to the determination of hemoglobin A2.
ONSIDERABLE EVIDENCE IS now available indicating that normal adult hemoglobin (hemoglobin A) is heterogeneous. Sharp and well-defined fractions of hemoglobin A, designated A2 and A3, were obtained by Kunkel (11), who used insoluble potato starch as a supporting medium for electrophoresis. Kunkel also was the first to measure quantitatively the amount of hemoglobin A2 and to correlate increases ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 16 شماره
صفحات -
تاریخ انتشار 1963